Information communication technology (ICT) that is used in instruction has attracted the attention of researchers with many studies having looked at the factors affecting how university instructors apply it in their teaching. Yet little is known about whether the gender of university teachers can impact their ICT use. This research relies on quantitative data from a survey of 428 teachers at the University of Ljubljana in Slovenia to explore differences in pedagogical ICT use in terms of gender. The results show that gender positively correlates with ICT use and that particular ICT tools and their pedagogical uses are more frequent among female teachers whereas males scored higher for certain ICT-related attitudes. This implies that gender may be viewed as a predictor of certain types of and attitudes to ICT use in university instruction.