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Title:
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ERROR SPOT FILTERING METHOD IN PROTEIN 2-DE IMAGE SPOT-MATCHING OPERATION |
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Author(s):
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Yanhua Jin , Jung Eun Shim , Won Suk Lee |
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ISBN:
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978-972-8924-30-0 |
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Editors:
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Nuno Guimarães and Pedro Isaías |
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Year:
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2007 |
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Edition:
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Single |
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Keywords:
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Proteomics, two-dimensional electrophoresis, spot-matching, filtering. |
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Type:
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Short Paper |
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First Page:
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499 |
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Last Page:
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503 |
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Language:
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English |
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Cover:
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Full Contents:
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click to dowload 
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Paper Abstract:
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The two-dimensional gel electrophoresis method (2-DE) is a widely used in Proteomics to separate thousands of proteins in a sample. By comparing the protein expression levels of proteins in a normal sample with those in a diseased one, it is possible to find a meaningful set of proteins for the disease. The major shortcomings of this approach are the inherent noises and irregular geometric distortions of spots in 2-DE images. Various experimental conditions can be the major causes of these problems. These problems eventually lead the protein analysis of samples to be incorrect conclusions. In order to minimize the influence of the problems, the proposed method in this paper performs the spot-matching operation of a set of gel images multiple times and segregates more reliable mapping results which can improve the accuracy of gel image analysis. The improved accuracy of the proposed method is analyzed by various experiments on real 2-DE images of human liver tissues. |
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